Abstract
Second order derivative spectrophotometry was used to improve the accuracy and reproducibility of cytochrome P-450 measurements in subcellular fractions obtained from the brain. This method allowed better resolution of the overlapping bands of cytochrome P-450 and other iron proteins, as well as a reduction of the effects of turbidity and non-specific components. Using this method, the cytochrome P-450 content of rat brain mitochondrial and microsomal fractions was measured to be 74.2 ± 2.3 and 5.9 ± 0.3 pmol/mg protein, respectively.
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