Abstract
The aim of this study was to establish, through a standard addition method, a convenient quantification assay for dipeptides (GY, YG, SY, YS, and IY) in soybean hydrolysate using 2,4,6-trinitrobenzene sulfonate (TNBS) derivatization-aided LC–TOF-MS. Soybean hydrolysate samples (25.0mgmL−1) spiked with target standards were subjected to TNBS derivatization. Under the optimal LC–MS conditions, five target dipeptides derivatized with TNBS were successfully detected. Examination of the standard addition curves, with a correlation coefficient of r2>0.979, provided a reliable quantification of the target dipeptides, GY, YG, SY, YS, and IY, in soybean hydrolysate to be 424±20, 184±9, 2188±199, 327±16, and 2211±133μgg−1 of hydrolysate, respectively. The proposed LC–MS assay is a reliable and convenient assay method, with no interference from matrix effects in hydrolysate, and with no requirement for the use of an isotope labeled internal standard.
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