Quantitative MAIPA: Comparison of different MAIPA protocols

Abstract

Introduction In a screening setting, maternal anti-HPA 1a antibody level has been found to be a good prognostic tool to identify newborns at risk for severe NAIT. Aim Identify the optimal MAIPA protocol for quantitation of anti-HPA 1a antibodies. Materials and methods Plasma were analysed for anti-HPA 1a antibodies using different monoclonal antibodies, lyophilized or fresh platelets and MAIPA protocols. Results The anti-HPA 1a antibody level varied significantly when different monoclonal antibodies were used. However, there was a strong correlation between maternal anti-HPA 1a antibody level and platelet count in the newborn. The sensitivity of the assay depended on the adopted MAIPA protocol. Conclusion Consistent tests results are of importance for the clinical impact of the test.