Quantitative light microscopic autoradiographic localization of binding sites labelled with [ 3H]vasopressin antagonist d(CH 2) 5Tyr(Me)VP in the rat brain, pituitary and kidney

Abstract

Binding sites for the vasopressin (VP) antagonist d(CH 2) 5Tyr(Me)VP, were located in various brain areas (e.g. the lateral septum, amygdala, choroid plexus and nucleus of the solitary tract) using light microscopic autoradiography. A number of areas (e.g. suprachiasmatic and arcuate nucleus, pineal gland) which previously showed no VP binding were labelled in the present study. The olfactory nucleus and ventromedial hypothalamic nucleus were not labelled. It therefore appears that d(CH 2) 5Tyr(Me)VP is capable of discriminating between VP and oxytocin binding sites and a more sensitive means of detecting VP binding sites than VP alone.