Quantitative LAMP and PCR Detection of Salmonella in Chicken Samples Collected from Local Markets around Pathum Thani Province, Thailand.

Virun Vichaibun,Panan Kanchanaphum

doi:10.1155/2020/8833173

Abstract

Salmonella is a bacterium that infects people when they consume contaminated food or liquids. To prevent humans from becoming ill, it is useful to have an efficient method of detecting Salmonella before the disease is passed on through the food chain. In this research, the efficiency of Salmonella detection was compared using the following four methods: conventional loop-mediated isothermal amplification (LAMP), PCR, quantitative LAMP (qLAMP), and qPCR. The artificial infection of chicken samples started with incubating of 10 mL of 108 CFU of S. typhimurium for 6 hr. and enriching for 2 hr. to represent real contamination of the samples. The results show that the sensitivity of Salmonella DNA detection in PCR, qPCR, LAMP, and qLAMP were 50 ng, 5 ng, 50 pg, and and 500 fg, respectively. Thirty samples of 10 g chicken were collected from 10 markets in Pathum Thani, Thailand; then, the infection was detected. The conventional LAMP, qLAMP, and qPCR methods detected Salmonella in all the chicken samples. However, the conventional PCR method detected Salmonella infection in only eight of the samples. Overall, the qLAMP method had the highest sensitivity of Salmonella DNA detection.

Highlights

Salmonellosis is a major foodborne infectious disease occurring worldwide, which is caused by the Gram-negative zoonotic pathogen, Salmonella [1]
The aim of this research is to compare the efficiency of loop-mediated isothermal amplification (LAMP), PCR, qualitative PCR, and qualitative LAMP by detecting Salmonella on chicken samples collected from markets around Pathum Thani province in Thailand
This results showed that the set of LAMP and PCR primers were specific-only Salmonella culture

Summary

Introduction

Salmonellosis is a major foodborne infectious disease occurring worldwide, which is caused by the Gram-negative zoonotic pathogen, Salmonella [1]. Salmonella is a member of the Enterobacteriaceae family, which has been divided into the following two species: S. enterica and S. bongori. S. enterica has been further divided into six subspecies that include over 2,500 serotypes [2]. Almost all Salmonella outbreaks have been broadly ascribed across multiple food categories, and many people have become infected by consuming contaminated food originating from animals (such as eggs, beef, poultry, and milk) [3]. The symptoms of patients diagnosed with typhoid fever, caused by Salmonella enterica serovar Typhimurium, are similar to other illnesses such as Leptospirosis and Streptococcus pneumoniae infection [4]. Powerful and more efficient methods of detecting Salmonella are still required

Objectives

Methods

Conclusion