Abstract
Microalgae cultivation on wastewater offers the dual benefit of lowering costs for feedstock production with simultaneous wastewater remediation. This study utilized biochemical and quantitative label-free proteomic approaches to evaluate the growth and proteomic response for diatom Phaeodactylum tricornutum cultivated on flushed dairy manure wastewater (DMW). Comparing several DMW dilutions (up to 60% DMW diluted in seawater) with a synthetic seawater medium indicates that biomass and lipid yields correlate with the starting nitrogen content of the DMW dilution. Phaeodactylum tricornutum cultivated on DMW exhibits elevated levels of polyunsaturated fatty acids (PUFAs), particularly docosapentaenoic acid (DPA, 22:5 n-3). Proteomic analysis revealed alterations in the regulations of proteins associated with protein metabolism, cellular signaling, transcription and translation, protein trafficking, and oxidative stress management pathways when comparing P. tricornutum cultivation on diluted DMW versus synthetic media, thus providing insights into how P. tricornutum reorganizes its proteome in response to a complex wastewater source.Graphical abstract
Highlights
Microalgal lipids are a promising renewable feedstock for the production of biodiesel and other high value co-products
Phaeodactylum tricornutum cultivated on 10%, 30%, and 60% dairy manure wastewater (DMW) for 15 days resulted in differences in cell density, biomass production, and chlorophyll a content when comparing DMW dilutions to each other and when compared to cultivation using synthetic medium (F2) over the same time period (Fig. 1a–c)
This study demonstrates the utility of dairy manure wastewater as a media source for P. tricornutum cultivation
Summary
Microalgal lipids are a promising renewable feedstock for the production of biodiesel and other high value co-products. Microalgae can grow in aquatic environments with varying salinities, possess higher growth and overall lipid production rates when compared to their terrestrial biofuel feedstock crop counterparts, and avoid conflicts over the use of terrestrial resources for fuel versus food production (Hannon et al 2010). The use of wastewater sources such as flushed dairy manure wastewater (DMW) for algal biomass production is a viable strategy for reducing microalgae cultivation costs while having potential benefit to the dairy industry (Chokshi et al 2016). Additional opportunities exist to further exploit the use of DMW as an alternate nutrient source for microalgae biomass production
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