Abstract
A CsCl centrifugation procedure was utilized to extract 85%–90% of the total cellular RNA from the immature rat uterus. This total RNA was deaggregated by treatment with sarkosyl and further fractionated by cellulose chromatography and acrylamide gel electrophoresis. Using these techniques, no specific RNA synthesis could be detected following in vitro incorporation of [ 3H]- and [ 14C]-adenosine into rat uterine RNA 15 min, 30 min and 5 h after in vivo 17β-estradiol administration.
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