Quantitative Investigation into the influence of intravenous fluids on human immune and cancer cell lines

Hande Karamahmutoglu,Meltem Elitas,Sumeyra Vural,Alara Altay

doi:10.1038/s41598-020-61296-5

Abstract

The effect of intravenous fluids (IVF) has been investigated clinically through the assessment of post-treatment reactions. However, the responses to IVF vary from patient-to-patient. It is important to understand the response of IVF treatment to be able to provide optimal IVF care. Herein, we investigated the impact of commonly used IVFs, Dextrose, NaCl and Ringer on different human cancer (HepG2 (liver hepatocellular carcinoma) and MCF7 (breast adenocarcinoma)) and immune cell lines (U937 (lymphoma) monocyte and macrophages). The effect of IVF exposure on single cells was characterized using hemocytometer, fluorescence microscopy and flow cytometry. Quantitative data on the viability and morphology of the cells were obtained. Our results emphasize that different IVFs demonstrate important differences in how they influence distinct cell lines. Particularly, we observed that the lactated ringer and dextrose solutions altered the viability and nuclear size of cancer and immune cells differently. Our findings present valuable information to the knowledge of cellular-level IVF effects for further investigations in IVF usage on diverse patient populations and support the importance and necessity of developing optimal diluents not only for drug stability but also for patient benefits.

Highlights

The effect of intravenous fluids (IVF) has been investigated clinically through the assessment of posttreatment reactions
Our study designed to interrogate the acute response of U937 monocytes, U937 monocyte-derived macrophages[20], HepG2 human liver hepatocellular carcinoma cells[21], and MCF7 human breast adenocarcinoma cells[22] when they were exposed to various IVF for 15 minutes
We observed the acute response of U937 monocytes, U937-derived macrophages, MCF7 and HepG2 cancer cells when we exposed them to IVF for 15 minutes

Summary

Introduction

The effect of intravenous fluids (IVF) has been investigated clinically through the assessment of posttreatment reactions. Patients with sickle cell disease might have localized microvascular obstruction during the vasso-oclusive crisis[16] Koustova and her co-workers quantified the production of reactive oxygen species from leukocytes when they were exposed to Lactated Ringer solution. Dr Banerjee and co-workers measured inflammatory cytokines and chemokines in response to TNF-α and IL-1β on alveolar pneumocyte line (A549) and concluded that hypertonic saline or hyperosmolar media disrupt cytokine signals at distinct intracellular steps[28]. This group previously reported that hypertonic saline inhibited TNF-α induced NF-κB activation in the pulmonary epithelium using immunofluorescent microscopy, flow cytometry, cell viability and western blot analysis[29]. Still most of the recent discussions focus on investigating large clinical trials to better define optimal fluid therapy in acute and critical care medicine[30,31,32,33,34]

Methods

Results

Conclusion