Abstract
Phototrophic microorganisms are promising resources for green biotechnology. Compared to heterotrophic microorganisms, however, the cellular economy of phototrophic growth is still insufficiently understood. We provide a quantitative analysis of light-limited, light-saturated, and light-inhibited growth of the cyanobacterium Synechocystis sp. PCC 6803 using a reproducible cultivation setup. We report key physiological parameters, including growth rate, cell size, and photosynthetic activity over a wide range of light intensities. Intracellular proteins were quantified to monitor proteome allocation as a function of growth rate. Among other physiological acclimations, we identify an upregulation of the translational machinery and downregulation of light harvesting components with increasing light intensity and growth rate. The resulting growth laws are discussed in the context of a coarse-grained model of phototrophic growth and available data obtained by a comprehensive literature search. Our insights into quantitative aspects of cyanobacterial acclimations to different growth rates have implications to understand and optimize photosynthetic productivity.
Highlights
Cyanobacteria are key primary producers in many ecosystems and are an integral part of the global biogeochemical carbon and nitrogen cycles
Steady-state specific growth rates in turbidostat mode were calculated from monitoring the optical density measured at a OUTPUT GAS DEHUMIDIFIER
The goal of this study was to close this gap with respect to knowledge and interpretation of key physiological parameters of the cyanobacterial model strain Synechocystis sp
Summary
Cyanobacteria are key primary producers in many ecosystems and are an integral part of the global biogeochemical carbon and nitrogen cycles. Due to their fast growth rates, high productivity and amenability to genetic manipulations, cyanobacteria are considered as promising host organisms for synthesis of renewable bioproducts from atmospheric CO2 (Al-Haj et al, 2016; Zavrel et al, 2016), and serve as important model organisms to understand and improve photosynthetic productivity.
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