Quantitative in vivo absorption spectra of phytoplankton: Detrital absorption and comparison with fluorescence excitation spectra1

Abstract

Spectral absorption of phytoplankton from cultures and natural samples was measured on filters with various optical setups including collimated and diffuse irradiation and measurements of wetted filters within an integrating sphere. In suspensions within an integrating sphere, specific absorption coefficients for laboratory cultures varied by a factor of only two.Measurements on filters yielded values dependent on filter load. Specific absorption coefficients derived from measurements of sample filters were considerably higher than values obtained from suspensions in an integrating sphere due to increased diffuseness of irradiance and to pathlength amplification by filter‐particle and particle‐particle interactions. Measured absorption of phytoplankton in the blue can be increased greatly by absorption of detritus, evident from absorption spectra of depigmented samples on filter. After subtracting detrital absorption, absorption spectra of phytoplankton are qualitatively similar to the corresponding quantum‐corrected fluorescence excitation spectra. The detritus‐corrected ratio of absorption at 440 vs. 675 nm shows average values between 1 and 1.5.Comparison with published values shows that specific in vivo absorption coefficients of phytoplankton are mostly overestimated as a result of the methodology applied. In the blue region of the spectrum, overestimation of phytoplankton absorption in field samples is possible if detrital absorption is neglected.