Quantitative in situ auswertung bandenförmig aufgetragener substanzen in der dünnschichtchromatographie

Abstract

Quantitative evaluation in situ of thin-layer chromatograms following non-quantitative sample application in the form of bands has been the subject of the experiments described in this paper. The technical advantage which application in the form of bands has over application in the form of spots with respect to measurement is stressed. Non-quantitative application may be taken into consideration when relating the quantity of the components to be analysed to the quantity of a so-called internal standard. Using an example from the field of clinical analysis—the analysis of cholesterin esters of the saturated fatty acids (cholesterin stearate) in human serum—details of the procedure are described. In another part of the paper the semi-quantitative determination in situ of other components of a compound with reference to the components analysed quantitatively is suggested. As an example a description is given of the determination of several cholesterin esters of unsaturated fatty acids in human serum which employs the cholesterin stearate calibration curve.In conclusion it is stated that the techniques described in the paper, i.e., the application in the form of bands and the use of an internal standard for reference, do not exclude employing procedures that do not require calibration samples. Experiments in which the transferability of the extinction from one plate to another is examined, have not yet been terminated. Moreover, it should be possible to use the described method in electrophoresis without further substantial limitations.