Abstract
A reversed-phase high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of ascorbic acid , free phenolic acids and flavonoids (catechin, rutin , quercetin, myrecetin, apigenin and Kaempferol) in four different solvent extracts of two wild edible leaves of viz. Sonchus arvensis and Oenanthe linearis, collected from North-eastern region in India . The chromatographic separation was carried out on Acclaim C 18 column (5 μm particle size, 250 x 4.6 mm) , Dionex Ultimate 3000 liquid chromatograph and detection was carried out at three different wave lengths (272 , 280 and 310 nm) using a mobile phase of acetonitrile and 1% aqueous acetic acid solution with gradient elution . The experimental results showed high amount of ascorbic acid in S. arvensis and O. linearis (1.2% and 2.3 % respectively) and gallic acid ( 0.02% and 0.06% respectively) in 1% aq. acetic acid extract of these two plants. The high percentage of recovery (96-103%), low coefficient of variation ( R2 > 0.99) and low limit of detection (LOD) and limit of quantitation (LOQ) confirm the suitability of the method for simultaneous quantification of ascorbic acid and all phenolic compounds in the two plants under investigation.
Highlights
The flavonoids are a large family of polyphenolic compounds synthesized by plants and structurally derived from the parent substance flavone
The constituents under investigation were identified by the recorded absorption spectra, which were comparable both for leaf extracts of S. arvensis, O. linearis and standard substances
The present study indicated the occurrence of large amount of ascorbic acid and gallic acid in the 1% aq. acetic acid extract of both S. arvensis and O. linearis leaves in comparison to other extractive media as in table 2
Summary
The flavonoids are a large family of polyphenolic compounds synthesized by plants and structurally derived from the parent substance flavone. Flavonoids present in fruits and leafy vegetables are thought to provide potential and versatile health benefits through radical scavenging and chelating activity. The in-vitro antioxidant activities of the flavonoids are due to their ability to reduce the free radical formation and exhibit several biological activities. Many studies have suggested that flavonoids like rutin, kaempferol, quercetin, apigenin etc. Are well-known for its antiinflammatory, anti-allergic, anti-thrombitic, hepatoprotective, anti-spasmodic and anticancer properties (Kumar, 2012). Each different fruits and leafy vegetables are capable to display different extent of antioxidant activities owing to the presence of varied amount of free phenolic and flavonol contents.
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