Quantitative gene expression of transcription factors RORγτ and FoxP3 in patients with liver cystic echinococcosis Turgun.

Abstract

Objective To investigate the mRNA expression and significance of transcription factors RORγτ and FoxP3 in peripheral mononuclear cells in patients with liver cystic echinococcosis, nethotis Ffity-four subjects were enrolled and derided into three groups： ealthy donor （HD, n = 20）, liver cystic echinococcosis （CE,n = 20）, ecuured cystic echinococcosis （RCE,n = g）. The expression of transcrition factors RORγτ and FoxP3 mRNA in peripheral mononuclear cells of patients with liver cystic echinococcosis was detected by real-time polymerase chain reaction （PCR）. Results The amplification efficiency of quantification of RORγτ and FoxP3 with real-time PCR was 90% -100%. The Ct in intra- and interassay was 〈 0. 20. As compared with HD group, the expression of RORγτ（ RORγτ copies/GAPDH copies） was decreased in CE and RCE groups （ t = 2. 91, t = 2. 79, P 〈 0.05 ） , but there was no statistically significant difference between CE and RCE groups （ t = 0. 56, P 〉 0.05 ）. Oppositely, the expression of FoxP3 （FoxP3 copies/GAPDH copies） was increased in CE and RCE groups as compared with HD group, but there was statistically significant difference （ t = 0.49, t = 0. 02, P 〉 0. 05 ）. The ratio of RORγτ and FoxP3 was obviously decreased in CE and RCE groups as compared with HD group （ t = 0. 33, t = 0. 35, P 〈0. 01 ）, but there was no significant difference between CE and RCE groups （ t = 0. 48, P 〉 0. 05 ）. Conclusion The real-time PCR assay for quantification of RORγτ and FoxP3 is efficient, special and productive. The increased expression of FoxP3 and decreased expression of RORγτ and ROR3,＇r/FoxP3 are related to special immunity during the pathogenesis of liver cystic echinococcosis, The decreased expression of RORγτ may play a major role and the RORγτ/FoxP3 expression ratio may be used as a more objective marker than RORγτ and FoxP3 alone for estimation of special immunovasion during the infection. Key words: Liver echinococcosis; T cells; Transcription factor; Immune evasion