Abstract
Objective To investigate the mRNA expression and significance of transcription factors RORγτ and FoxP3 in peripheral mononuclear cells in patients with liver cystic echinococcosis, nethotis Ffity-four subjects were enrolled and derided into three groups: ealthy donor (HD, n = 20), liver cystic echinococcosis (CE,n = 20), ecuured cystic echinococcosis (RCE,n = g). The expression of transcrition factors RORγτ and FoxP3 mRNA in peripheral mononuclear cells of patients with liver cystic echinococcosis was detected by real-time polymerase chain reaction (PCR). Results The amplification efficiency of quantification of RORγτ and FoxP3 with real-time PCR was 90% -100%. The Ct in intra- and interassay was 〈 0. 20. As compared with HD group, the expression of RORγτ( RORγτ copies/GAPDH copies) was decreased in CE and RCE groups ( t = 2. 91, t = 2. 79, P 〈 0.05 ) , but there was no statistically significant difference between CE and RCE groups ( t = 0. 56, P 〉 0.05 ). Oppositely, the expression of FoxP3 (FoxP3 copies/GAPDH copies) was increased in CE and RCE groups as compared with HD group, but there was statistically significant difference ( t = 0.49, t = 0. 02, P 〉 0. 05 ). The ratio of RORγτ and FoxP3 was obviously decreased in CE and RCE groups as compared with HD group ( t = 0. 33, t = 0. 35, P 〈0. 01 ), but there was no significant difference between CE and RCE groups ( t = 0. 48, P 〉 0. 05 ). Conclusion The real-time PCR assay for quantification of RORγτ and FoxP3 is efficient, special and productive. The increased expression of FoxP3 and decreased expression of RORγτ and ROR3,'r/FoxP3 are related to special immunity during the pathogenesis of liver cystic echinococcosis, The decreased expression of RORγτ may play a major role and the RORγτ/FoxP3 expression ratio may be used as a more objective marker than RORγτ and FoxP3 alone for estimation of special immunovasion during the infection. Key words: Liver echinococcosis; T cells; Transcription factor; Immune evasion
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.