Abstract
A rapid, sensitive, and specific fluorometric TLC procedure for the estimation of hydroflumethiazide in human biological fluids is described. The drug was removed from plasma in a single extraction step and applied to a TLC plate of silica gel. After development, the plate was scanned in a spectrodensitometer equipped to measure fluorescence below 400 nm. Quantitation was achieved by comparing the areas under the peaks obtained from the unknowns to those obtained from standards applied to the same plate. The limit of detection was 10 ng of drug/ml of plasma. A mean recovery value of 99.7% was obtained when the drug was extracted from spiked human plasma samples, while 97.5% of drug was recovered from spiked human urine samples. To test the validity of the method, a beagle dog was given an intravenous dose of hydroflumethiazide, and postadministration blood and urine samples were collected and analyzed.
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