Quantitative Fluorescence Microscopy on SARS-CoV-2

Abstract

Severe acute respiratory coronavirus-2 (SARS-CoV-2) is the causative agent of Coronavirus-19 Disease (COVID-19) and remains a severe public health threat. SARS-CoV-2 particles contain four structural proteins - i.e., membrane (M), nucleocapsid (N), spike (S) and envelope (E). Early in virus assembly, the M, S and E proteins are inserted into the rough endoplasmic reticulum (ER). Upon their transport to the ER- Golgi intermediate compartment (ERGIC), there is interaction with N - which initiates particle biogenesis.