Abstract
We developed a method to evaluate the potency of Na+/Ca2+ exchanger (NCX) inhibitors with epifluorescence microscopy and kinetic analysis in NCX1-transfected HEK 293 cells loaded with the Ca2+-sensitive fluorescent dye, fura-2. Changing the extracellular solution to low Na+ solution induced an increase in cytoplasmic Ca2+ and return to normal solution resulted in a decrease towards initial level. The early phase of the increase in cytoplasmic Ca2+ concentration could be fit into a linear function and its slope was concentration-dependently decreased by NCX inhibitors, SEA0400 and KB-R7943.
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