Abstract
A method is described for the determination of nonesterified fatty acids in plasma. Extraction is at least 98% efficient, and losses during subsequent stages are corrected for by the use of an internal radioactive standard. The method is suitable for reference purposes rather than for routine determinations. Higher values are obtained than by other methods of analysis, and it is suggested that some plasma fatty acids remain protein-bound after normal methods of extraction.
Highlights
The new method has been compared with a colorimetric method (l), and the results confirm our belief that existing methods do not give a true value for plasma nonesterified fatty acids (NEFA)
T o check that no stage of the standard method caused hydrolysis of lipids, leading to afalsely high value for the final observed NEFA level, thestandard method was applied to apreparation of typicalplasma lipids other than NEE’A
NEFA levels in a number of plasma samples determined in parallel by the method described in this paper and by a colorimetric method [1]
Summary
Summary A method is describedforthedetermination of nonesterified fatty acids in plasma. IN CONNECTION WITH work on methods for the determination of nonesterified fatty acids (free fattyacids) in plasma, we requiredan absolutemethod for reference purposes. Even in the absence of protein only about 86y0 of apalmiticacid standardappeared in theorganicphase,andit seems probable that plasma free fatty acids which are virtually all protein-bound [3] will be still less efficiently extracted. Uncertainties of this kind in existing methods made it necessary to devise a quantitatively reliable method, and we present details of such a method in this paper. The new method has been compared with a colorimetric method (l), and the results confirm our belief that existing methods do not give a true value for plasma NEFA
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
