Abstract
This study aims to quantitatively analyze cellular uptake following local ultrasound (US)-mediated cell permeabilization. A 2μM cell-impermeable dye Sytox Green was co-injected with 3 × 10(7) microbubbles in the presence of C6 rat glioblastoma cell monolayer in total volume of 10ml. A 5.8-mm diameter mono-element US transducer was positioned at a distance of 8mm to the Opticell® membrane. Acoustical pressure of pulsed US was varied from 0.62MPa peak-to-peak (p-p) to 1.25MPa p-p. Large field of view (FOV = 15 × 15mm) 22 × 22 mosaic acquisitions were done under epifluorescence Leica DMR microscope and analyzed in Metamorph software to evaluate cell density as well as model drug uptake percentage. The size of acoustical field of the transducer closely matches the spatial pattern of the model drug internalized into the cells by US. Maximum of uptake percentage (42 ± 15%) was found at 0.88MPa p-p. Spatial aspect of US-mediated model drug uptake has been quantitatively evaluated on adherent cells using robust 2D-mapping approach.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
