Abstract
Previous studies have shown the feasibility of assessing qualitative CBV measurements in the angiography suite by using FPD-CBCT systems. We have investigated the correlation of FPD-CBCT CBV lesion volumes to the infarct volume. Unilateral strokes were created in 7 adult dogs. MR imaging and FPD-CBCT data were obtained after MCA occlusion. FPD-CBCT CBV and ADC maps were generated for all subjects. The animals were sacrificed immediately following the last imaging study to measure infarct volume on histology. The reliability of FPD-CBCT-based lesion volume measurements was compared with those measured histologically by using regression and Bland-Altman analysis. The best correlation (R(2) = 0.72) between lesion volumes assessed with FPD-CBCT and histology was established with a threshold of mean healthy CBV - 2.5 × SD. These results were inferior to the correlation of lesion volumes measured with ADC and histology (R(2) = 0.99). Bland-Altman analysis showed that the agreement of ADC-derived lesion volumes with histology was superior to the agreement of FPD-CBCT-derived lesion volumes with histology. We correlated FPD-CBCT measurements of CBV and MR ADC lesion volumes with histologically assessed infarct volume. As expected, ADC is a very accurate and precise method for determining the extent of infarction. FPD-CBCT CBV lesion volumes are correlated to the size of the infarct. Improvement of FPD-CBCT image quality provides an opportunity to establish quantitative CBV measurement in the angiography suite.
Highlights
MethodsUnilateral strokes were created in 7 adult dogs. MR imaging and FPDCBCT data were obtained after MCA occlusion
AND PURPOSE: Previous studies have shown the feasibility of assessing qualitative CBV measurements in the angiography suite by using FPD-CBCT systems
BlandAltman analysis showed that the agreement of ADC-derived lesion volumes with histology was superior to the agreement of FPD-CBCT-derived lesion volumes with histology
Summary
Unilateral strokes were created in 7 adult dogs. MR imaging and FPDCBCT data were obtained after MCA occlusion. The animals were sacrificed immediately following the last imaging study to measure infarct volume on histology. The reliability of FPD-CBCT-based lesion volume measurements was compared with those measured histologically by using regression and Bland-Altman analysis. Stroke Preparation Experiments were approved by the Institutional Animal Care and Use Committee of the University of Massachusetts. Stroke was induced in 7 purpose-bred canines (beagles, mean weight of 10.0 kg) by injection of an autologous blood clot into the left or right, randomly selected, ICA under fluoroscopic guidance by using a 5F catheter, as previously described.[15] Thrombin-induced autologous clot (4 National Institutes of Health unit/mL blood) was prepared in silicone tubing 1 day before the experiment. Animals were anesthetized by intramuscular injection of acepromazine (0.06 mg/kg), glycopyrrolate (0.01 mg/kg), and thiopental (15.0 mg/kg). Physiologic monitoring, including heart rate, blood pressure, arterial oxygen saturation, temperature, end-tidal CO2, blood glucose, and blood gases, was performed and recorded every 15 minutes during the procedure
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