Abstract
SUMMARY The inhibition tests of leukocyte migration and of target cell destruction have been compared for their sensitivity for the detection of transplantation antigens. The migration inhibition test was performed with spleen cells of C57BL/6J animals which had been immunized with Sal cells of the A strain. Inhibition of target cell destruction was carried out with rabbit antisera, using guinea pig complement and 51Cr-labeled target lymphocytes; the inhibitory antigen was the crude membrane-lipoprotein preparation isolated from the spleens and lymph nodes of normal A/J animals. Significant inhibition of leukocyte migration was obtained with the antigen preparation at a concentration of 100 µg/ml or higher. The inhibition of target cell lysis depended on the cytotoxic titer of the antiserum; at 71 and 56% cytotoxic titers, complete inhibitions were obtained with 100 and 25 /µg of antigen, while 50% inhibitions were obtained with 70 and 14 /µg of antigen, respectively.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
