Abstract
The placental isoenzyme of alkaline phosphatase is characterized by heat stability (56° C for 30 min). This paper describes a new technique for the forensic diagnosis of pregnancy from blood stains, based on the fact that in serum of pregnant women in the latter half of pregnancy the heat-stable alkaline phosphatase activity rises markedly. In this study the total and heat-stable alkaline phosphatase activities were quantitatively assayed in blood stains of various origins by the method of Bessey-Lowry-Brock with the use of disodium p-nitrophenyl phosphate as substrate. Our results indicate that the principle herein reported may be helpful in the diagnosis of pregnancy from blood stains.
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Schedule a callMore From: Zeitschrift fur Rechtsmedizin. Journal of legal medicine
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