Abstract
A normal phase high-performance thin-layer chromatographic method was developed and validated for simultaneous estimation of Artesunate and Amodiaquine HCL in bulk and pharmaceutical formulations. Chromatographic separation was achieved using precoated silica gel aluminum plate G60 F254 and a mixture of acetonitrile/water/ammonia (30%) (8:1.4:0.4, v/v/v) as mobile phase. Quantitative analysis of Artesunate and Amodiaquine HCL was carried out using Camag TLC Scanner 3. The method was linear between 100 and 600ng/spot for Artesunate, and 50 and 300ng/spot for Amodiaquine HCL. The detection limit for Artesunate and Amodiaquine was found to be 25 and 12.5ng/spot, respectively, whereas quantitation limit was 60ng/spot for Artesunate and 30ng/spot for Amodiaquine HCL. The method demonstrated good and consistent recoveries (98.07–99.88%for Artesunate and 99.28–100.94%for Amodiaquine HCL) with low inter-day and intra-day relative standard deviation. The validated method was applied successfully to quantify Artesunate and Amodiaquine HCL simultaneously in pharmaceutical formulations. The method was found to be precise, sensitive, and accurate for the simultaneous determination Artesunate and Amodiaquine HCL in bulk and pharmaceutical formulations.
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