Abstract
A new quantitative approach to investigate the effect of topically applied anti-inflammatory agents is proposed. An external ocular inflammation was produced in the rat by topical instillation of ocular irritants. The method requires a short time and is based upon quantitatively determining the degree of edema by weighing the eyelid. This end-point can be evaluated objectively, in contrast to the subjective results obtained by many widely used methods. Hydrocortisone, triamcinolone acetonide, triamcinolone, fluocinolone acetonide, and dexamethasone were tested. Dose–response relationships were obtained, and relative potencies were determined by parallel-line assay, using hydrocortisone as a reference standard. Fluocinolone acetonide, the most potent steroid studied, had a topical relative potency of 2745; triamcinolone, the least potent, had a topical relative potency of 4.9. The systemic relative potency was also investigated. The results obtained show that the experimental model of external ocular inflammation appears to be a reliable method for quantitative assay of topical anti-inflammatory agents.
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