Abstract
We propose a method for estimating the stiffness of bio-specimens by measuring their linear retardance properties under applied stress. For this purpose, we employ an epi-illumination Mueller matrix microscope and show the procedures for its calibration. We provide experimental results demonstrating how to apply Mueller matrix data to elastography, using chicken liver and chicken heart as biological samples. Finally, we show how the histograms of linear retardance images can be used to distinguish between specimens and quantify the discrimination accuracy.
Highlights
The determination of tissue mechanical properties is of large interest in clinical applications for the diagnosis and detection of many diseases, since these properties vary with the pathological condition of the tissue
We have demonstrated a method to determine the elastographic parameters in terms of a stress-optic modulus and a stress-retardance sensitivity coefficient for differentiation of biological samples
Linear retardance images are retrieved from the Mueller matrix elements and are shown for the biological samples including chicken liver and chicken heart
Summary
The determination of tissue mechanical properties is of large interest in clinical applications for the diagnosis and detection of many diseases, since these properties vary with the pathological condition of the tissue. After using the rubber sample to verify the accuracy of measuring Young’s modulus using the Mueller matrix microscope system, we establish an empirically determined linear relationship between the sample linear retardance in reflection and the applied stress. This relationship is described by what we call the sample’s “stress-retardance sensitivity coefficient” and by comparing the stress-retardance sensitivity coefficient of different tissues, we show that it is possible to discriminate tissue types even when they appear same to the eye
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