Abstract
We measured the contents of Zn protoporphyrin IX (ZPP), heme and protoporphyrin IX (PPIX) in Parma ham by simultaneous analysis using high-performance liquid chromatography (HPLC). Extraction with ethyl acetate–acetic acid (4:1) was suitable for the quantitative analysis of ZPP. The contents of heme, ZPP and PPIX in Parma ham were 15.0–29.9, 27.7–47.0 and 0.4–1.1 μg/g, respectively, and total content of porphyrin was 43.7–76.6 μg/g. The amount of ZPP in Parma ham was larger than that of heme, and ZPP accounted for 60–70% of all porphyrins.
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