Abstract
A HPLC/ICP-QQQ-MS method for quantifying the health-relevant sulfur species ergothioneine was developed and applied to human cells and mushrooms.
Highlights
Ergothioneine, a natural sulfur-containing histidine derivative, was rst isolated in 1909 from Claviceps purpurea, an ergot fungal infection of rye grain.[1]
Due to these advantages HPLC/ICPMS is a common technique for elemental speciation analysis it has not been widely applied to sulfur species, mainly because of isobaric interferences on the main sulfur isotope 32S+
With a view to future investigations comparing the biological chemistry of sulfur and selenium, we demonstrate the potential of the method to provide simultaneous measurements of selenium and sulfur species by a rst analysis of aqueous extracts of oyster mushrooms
Summary
Ergothioneine, a natural sulfur-containing histidine derivative, was rst isolated in 1909 from Claviceps purpurea, an ergot fungal infection of rye grain.[1]. We present here a HPLC/ICP-QQQ-MS method for the quantitative determination of the health-relevant sulfur compound ergothioneine and its application to cell culture media and cell pellets.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
