Abstract
Quantitative determination of the sulfated glycoproteins present in the secretion fluid was performed. After digestion of the specimen with pronase in order to convert glycoproteins to glycopeptides, sialogylcopeptides and sulfated glycopeptides) by two-dimensional electrophoresis on cellulose acetate membrane [(1986) J. Biochem. Biophys. Methods 12, 239–246]. After staining with alcian blue, the spot of sulfated glycopeptide on the cellulose acetate membrane was cut out, and then only the dye bound to the sulfated glycopeptide was extracted with a 5% cetylpyridium chloride solution at 100% for 15 min. The extract was then measured by absorbance at 615 nm an authentic sulfated glycopeptide as a standard. This method facilitated the determination of sulfated glycopeptides, which were separated from other acidic glycans, within the range 0–25 μg.
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