Abstract
Quantitative assays for prostaglandins (PG) E 1 and PGF 1α are described using [3,3,4,4,5,6- 2H 6]labeled prostaglandins as carriers and methyl ester- O-methyloxime-acetate (PGE 1) and methyl ester-acetate (PGF 1α) derivatives for gas - liquid chromatography/mass spectrometric analysis. Thin-layer argentation chromatography was used to separate PGE 1 from PGE 2 and 13, 14-dihydro-PGE 2. These latter compounds, which do not separate from PGE 1 using conventional thin-layer chromatography or under the gas - liquid chromatographic conditions used, can significantly interfere with the quantitative analysis of PGE 1. The method described prevents this interference and is therefore suitable for the accurate analysis of PGE 1 in biological samples containing a high concentration of PGE 2 and/or 13, 14-dihydro-PGE 2.
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