Abstract
Abstract A gas chromatographic method is described for the direct determination of physostigmine base from a solution of physostigmine salicylate. The method involves isolation of physostigmine salicylate from an aqueous solution by a freeze drying process, subsequent conversion to a trimethylsilyl (TMS) derivative and gas liquid chromatography. The trimethylsilyl salt dissociates on injection into the flash vapourizer inlet of a gas Chromatograph, and the physostigmine-TMS and salicylic acid-TMS derivatives are eluted separately. Due to the specificity of the silylation reaction it is possible to determine by gas chromatography the alkaloid content in the presence of its degradation products. A modification of the method was necessary for the assay of a commercial formulation containing certain additives.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
