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Abstract
A method based on thin-layer chromatography was devised for the determination of microgram quantities of the phospholipids. This procedure was used to estimate both the quantity and the radioactivity of the ethanolamine plasmalogens by taking advantage of the fact that the α,β-unsaturated ether bond of these compounds is hydrolyzed in the presence of mercury salts. The conditions of this reaction were investigated to ensure that complete hydrolysis took place and that the ester phospholipids were not affected. Under these conditions, estimation of the lysophosphatidyl ethanolamine produced permitted accurate quantitation of the phosphatidal ethanolamine in the lipid mixture. With this method values were obtained for the phosphatide composition of whole rat brain, and the phosphatidal ethanolamine content of a number of other tissues. The assay procedure can also be applied to other chromatographic systems.
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