Abstract
A procedure is described for the microdetermination of fucose, galactose, and mannose residues in glycoproteins and glycopeptides by direct densitometry of thin-layer chromatograms. A column of ion-exchange resin is employed for the removal of peptides, amino acids, amino sugars, and inorganic ions from hydrolyzates prior to chromatography. Separation is carried out on 250 μ cellulose layers, and aniline phthalate reagent is used for color development. Accuracy is about ±10% for a single determination. Application of the method to the analysis of human α 1 acid glycoprotein and ovine luteinizing hormone is described.
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