Abstract
A quantitative determination of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine from dog lungs by means of the corresponding diacyl glycerol trimethylsilyl ether derivative with 1,2-dimyristoyl- sn-glycero-3-phosphocholine as internal standard was carried out by gas-liquid chromatography with glass capillary columns. The individual diacyl glycerol species were identified by using a combination of gas-liquid chromatography and mass spectrometry. In a total of 32 determinations percent deviations from the mean value between ± 2.0 and ± 4.4% were achieved. By using glass capillary columns instead of conventional columns the time required for an analysis was reduced to ca. 30% and separation was considerably improved. Thus the diacyl glycerol species from PC-32 were separated according to both the 1,2 and 1,3 positional isomerism and the degree of saturation of the acyl radicals.
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