Abstract
A method is developed for simultaneous determination of lactic acid and glycerol in wine. The method is based upon the solubility of barium lactate and glycerol in 70-80% ethanol. The wine samples are treated with barium hydroxide and extracted with ethanol. Following evaporation of an aliquot of the ethanol extract, trimethylsilyl derivatives of lactic acid and glycerol are prepared and gas chromatographed. The standard error for lactic acid and glycerol were calculated and found to be respectively ±0.005 and ±0.02 g/100 ml.
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