Abstract
Novel adsorptive stripping square-wave voltammetric method as well as a new high-pressure liquid chromatographic method for direct determination of glycyrrhizinic acid in dosage pharmaceutical preparation, used against virus infections, have been developed. Glycyrrhizinic acid is an electrochemically active compound, which undergoes irreversible reduction on a mercury electrode surface in an aqueous medium. Its redox properties were studied thoroughly by means of square-wave voltammetry, as one of the most advanced electroanalytical technique. The voltammetric response depends mainly on the pH of the medium, composition of the supporting electrolyte, as well as the parameters of the excitement signal. It was also observed that the voltammetric properties strongly depend on the accumulation time and potential, revealing significant adsorption of glycyrrhizinic acid onto the mercury electrode surface. Upon this feature, an adsorptive stripping voltammetric method for quantitative determination of glycyrrhizinic acid was developed. A simple, sensitive and precise reversed phase HPLC method with photodiode array UV detection has also been developed, mainly for comparison and conformation of the results obtained with the voltammetric method.
Highlights
IntroductionGlycyrrhizinic acid (GA) is a saponin extracted from Glycyrrhiza glabra root
It was observed that the voltammetric properties strongly depend on the accumulation time and potential, revealing significant adsorption of glycyrrhizinic acid onto the mercury electrode surface
Glycyrrhizinic acid (GA) is a saponin extracted from Glycyrrhiza glabra root
Summary
Glycyrrhizinic acid (GA) is a saponin extracted from Glycyrrhiza glabra root. According to its chemical structure, it belongs to penthacyclical triterpenoid saponins, products of β-amyrin [1]. GA and its aglycon 18β glycyrrhetinic acid are widely used against bacterial and virus infections, as antiflogistics, expectorants, corrigens and as components with mineralocorticoid effect [2]. Immunoassays (ELISA and RIA techniques) were used for determination of the glycyrrhizinic acid [15]. The general purpose of the work was to develop simple, precise and accurate methods for quantification of glycyrrhizinic acid in pharmaceutical dosage forms
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