Abstract
A simple, sensitive, and rapid method for quantitative determination of free phytosterols (cholesterol, ergosterol, stigmasterol, and beta-sitosterol) in tobacco leaves by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) has been developed. The separation of the four phytosterols was realized on a C18 reversed-phase column within 6.0 min. A triple quadrupole mass analyzer API 4000 MS/MS System was evaluated for its performance in the study; the identification and quantitation of analytes were performed in the positive ion multiple reactions monitoring mode (MRM). Satisfactory recovery ranged from 86.3% to 106.4% at middle quality concentration (MQC) levels in tobacco leaves. The intra-day precision and inter-day precision accuracy of the method were between 0.43% and 2.42%. The limit of quantification (LOQ) was 4.8-9.7 ng/mL. The applicability of this newly developed method was demonstrated by analysis of free phytosterols in tobacco leaves samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
