Abstract
Losartan potassium, Valsartan , Telmisartan and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. A gradient HPLC method was developed for the assay of several ARAII in presence of Hydrochlorothiazide. The method was performed by reversed phase high performance liquid chromatography using a mobile phase 0.025 M potassium dihydrogen phosphate (pH 6.0): acetonitrile =80:20% with detection at 220 nm on an ACE C18 column (250 mm × 4.6 mm, 5 μm) at flow rate 1.5 ml/min in a gradient manner. The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation.
Highlights
Angiotensin antagonists are the first major innovation in essential hypertension management as a first-line treatment
Literature review revealed that USP described reversed phase (RP)-HPLC methods for assay of Losartan potassium, Valsartan and ion pair HPLC for Irbesartan and Telmisartan
One method has been established to assay ARAII and hydrochlorothiazide by electrophoresis [20] but this technique is less available in pharmaceutical companies than HPLC-UV and more expensive so, it is preferable that the developed method is HPLC-UV, the most spreadable apparatus in pharmaceutical companies
Summary
Angiotensin antagonists are the first major innovation in essential hypertension management as a first-line treatment. Telmisartan, Irbesartan and Valsartan are highly selective, non-peptide angiotensinII receptor antagonist s (ARA-II). They are effective agents for the treatment of hypertension and heart failure either alone or together with diuretics or recently with other antihypertensive drugs [2]. It is necessary to develop a validated analytical method for assay of ARAII in combination with hydrochlorothiazide in its pharmaceutical preparations. Literature review revealed that USP described RP-HPLC methods for assay of Losartan potassium, Valsartan and ion pair HPLC for Irbesartan and Telmisartan. Some methods have been published for simultaneous determination of studied ARA-II-drugs separately in combination with hydrochlorothiazide in its pharmaceutical preparations [5,6,7,8,9,10,11,12,13,14,15]. Our recent method is characterized by a simplicity, accuracy, preciseness and sensitivity
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