Abstract
Fibrinogen can transform fibrin through an agglutination reaction, finally forming fibrin polymer with grid structure. The density and viscosity of the reaction system changes drastically during the course of agglutination. In this research, we apply an independently-developed piezoelectric agglutination sensor to detect the fibrinogen agglutination reaction in patients with coronary heart diseases. The terminal judgment method of determining plasma agglutination reaction through piezoelectric agglutination sensor was established. In addition, the standard curve between plasma agglutination time and fibrinogen concentration was established to determinate fibrinogen content quantitatively. The results indicate the close correlation between the STAGO paramagnetic particle method and the method of piezoelectric agglutination sensor for the detection of Fibrinogen. The correlation coefficient was 0.91 (γ = 0.91). The determination can be completed within 10 minutes. The fibrinogen concentration in the coronary heart disease group was significantly higher than that of the healthy control group (P < 0.05). The results reveal that high fibrinogen concentration is closely correlated to the incurrence, development and prognosis of coronary heart diseases. Compared with other traditional methods, the method of piezoelectric agglutination sensor has some merits such as operation convenience, small size, low cost, quick detecting, good precision and the common reacting agents with paramagnetic particle method.
Highlights
Fibrinogen (Fib), a blood coagulating protein with its highest content in plasma, is one of the acute phase proteins, synthesized by liver cells and megakaryocytes
As the agglutination reaction proceeded, Fib changed into fibrous protein F1 monomer and complex, and the density and viscosity of the reaction system increased constantly
With deposit of blood clots, the crystal frequency decreases, and the turning point of frequency is the terminal of agglutination reaction
Summary
Fibrinogen (Fib), a blood coagulating protein with its highest content in plasma, is one of the acute phase proteins, synthesized by liver cells and megakaryocytes. Fib is closely associated with consumption coagulopathy, liver diseases, nephropathy syndrome, cardiovascular diseases, diabetes and malignant tumors, etc. Previous reports often focused on hemorrhagic diseases, thromboembolic diseases and recurrent spontaneous abortions caused by genetic variation of Fib [7,8]. More attention needs to be paid to Fib determination in the clinical field. There are three types of Fib determination methods—physical-chemical methods (thermal/salt precipitation method, etc.), congealable protein methods and immunology methods [914]. The thermal/salt precipitation method possesses the largest application scope, specificity is not ideal because the results are influenced by other proteins in the specimen. The congealable protein method reflects the blood agglutination function of
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