Quantitative Determination of Drugs by using Chloramine-T and Methyl orange Couple: A spectrophotometric study

Abstract

Simple, sensitive, accurate and precise method for quantitative determination of each of five drugs viz., Esomeprazole (ESO), Ciprofloxacin (CIP), Lomefloxacin (LOM), Sumatriptan, (SUM), Domperidone (DOM) have been developed and validated. The method is based on oxidation of drug by Chloramine-T (excess) and subsequent determination of unreacted Chloramine-T using methyl orange as analytical tool which maximally absorbs at 510nm. Beer's law is obeyed in the concentration range of 1.5–4.5, 2.0–14, 2.5–17.5, 2.0–16, 1.5–10.5 μg ml−1 for ESO, CIP, LOM, SUM and DOM respectively. Six replicates were performed to assess the precision and accuracy is determined by analyzing samples of known concentration. % recovery and % RSD were calculated which showed excellent accuracy. The spectral parameters like limits of Beer's law, sandal sensitivity, molar absorptivity, slope, intercept and regression equation have been evaluated. The validation parameters like LOD, LOQ together with statistical parameters like t-test and F-test have also been carried out.