Abstract
ABSTRACT A simple and sensitive extraction-spectrophotometric method in the visible region is described for the determination of diazepam in bulk sample and pharmaceutical formulations. The method is based on 1 : 1 ion-association complex formation with the acidic dye, bromocresol green at pH 3.5, which is extractable into chloroform from the aqueous phase. Spectrophotometric measurement was done at 410 nm. Different variable affecting the reaction were studied and optimized. Beer's law is obeyed in a general concentration range of 2–60 µgċmL−1 for the investigated complex. Sandell sensitivity for 0.001-absorbance unit is 57 ngċcm−2. The proposed method could be applied successfully to the determination of diazepam in tablets and injections with good accuracy and precision. The results were compared with the official method. By using the method, diazepam was recovered from a blood serum sample.
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