Abstract
An in vivo fluorimetric method for selectively determining the concentration of cyanobacteria (blue-green algae) in mixed phytoplankton communities was developed. Excitation wavelengths of 620 nm and 440 nm were applied, the former for detecting the cyanobacteria themselves, and the latter for subtracting the interference due to eukaryotic algae present in the sample. The emission fluorescence of the cyanobacteria and eukaryotic algae was measured at 645 nm and 680 nm, respectively. In the range of 0.01 to 10 μg phycocyanin ml −1 a linear relationship between fluorescence intensity and cell concentration was established for all the species examined: Microcystis aeruginosa, Phormidium tenue, Anabaena cylindrica and Spirulina platensis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
