Abstract
The determination of E, NE, and DA in human red blood cells are described. The catecholamines are extracted from the red blood cells, derivatized and analyzed by gas-liquid chromatography with the dual hydrogen flame detector. The minimum volume of red blood cells used in this study was 0.5 ml. The detection limit for the catecholamines with the hydrogen flame detector was in the picogram range. Dopamine, which constitutes the major source of interference in the commonly used methods, does not interfere with the separation of E and NE. The advantage of using GLC is that the three catecholamines can be separated on the same chromatogram. In the commonly used fluorometric method, the absorption spectra of E, DA, and NE is the same. The catecholamines can only be separated by pH adjustment, which makes their measurements very time-consuming.
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