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Abstract
1. Using high performance liquid chromatography with electrochemical detection we have assayed serotonin (5-HT), octopamine (OA). dopamine (DA), noradrenaline (NA), adrenaline (A) and dihydroxyphenylalanine (DOPA) in the CNS of adult and larval blowflies, Calliphora erythrocephala. 2. The adult CNS was also partitioned and optic lobes, brain minus optic lobes and ventral ganglia were assayed separately. 3. In the adult and larval CNS all the assayed compounds except NA and A were detected. 4. In the adult flies the amines and DOPA occurred within the range of 0.29–4.0 pmol per tissue. 5. In the larval CNS DOPA was present in large amounts, 9.8 pmol per tissue, whereas the others were in the range of 0.51–1.1 pmol per tissue. 6. Measured in pmol per tissue, the adult optic lobes contained less of all the compounds than the remaining brain and the ventral ganglia less than the entire brain (except for DOPA). 7. The larval CNS (including the precursors of imaginai neurons) contained less 5-HT, OA and DA than the adult, but much more DOPA.
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Schedule a callMore From: Comparative Biochemistry and Physiology. Part C, Comparative Pharmacology
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