Abstract
Abstract A method was developed for quantitative analysis of bile acids by chromatography and densitometry. Within the range of experimental error, the results of cholic and chenodesoxycholic acid determinations on biological samples agreed with those obtained by the spectrophotometric method of Ganshirt1. Reproducibility was influenced by the uniformity of the plate-coating and of the spraying, by width of channels, and by spot size. Since several samples may be run simultaneously and it is unnecessary to locate and remove them from the plates for spectrophotometric analysis, this method has advantages over methods currently employed for bile acid analysis.
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