Abstract
Extraction, isolation and characterization of Andrographis paniculata (A.p.) products were developed. Three natural diterpenes compounds were obtained and one was used for chemical modifications. Evaluation of their inhibition of TNFα induced NFκB transcriptional activity. A rapid analytical method for the determination and quantitation of three diterpenoid lactones (andrographolide 1, didehydroandrographolide 2, neoandrographiside 3) found in A. paniculata extracts was investigated. After some optimizations on column type and injection solvent, the separation was achieved in 9 min on a monolithic Chromolith Performance RP18e column (100 mm × 4.6 mm ID, 2 µm), with a gradient solvent system of water and methanol, UV detection at 220 nm and ELSD detection. The method was proved to be suitable for the quantitation of these three diterpenes in four different commercial Andrographis dietary supplements. The anti-inflammatory activities of a mixture of known composition have been evaluated showing differences in activity depending on the relative ratio of various diterpenes and also a possible synergic activity for some of them.
Highlights
Andrographis paniculata (Acanthaceae) (A.p.) is widely used as medicinal herb in traditional medicine [1,2,3]
In order to understand the mechanism of inhibition of NFκB by andrographolide, andrographolide 1 was treated
B and C where the quantity of 2 is not negligible, the inhibitory activity increases. These results could explain those reported in the literature [31], showing that there were other compounds than andrographolide in A. paniculata extracts that act as an immunostimulant, and we proved that there was a complementary activity of 2 at the studied concentrations
Summary
Andrographis paniculata (Acanthaceae) (A.p.) is widely used as medicinal herb in traditional medicine [1,2,3]. Diterpenoid lactones isolated from these extracts have been the subject of intensive investigations and are reported to exhibit a wide spectrum of biological activities, including antibacterial [4], anti-inflammatory [5,6,7,8], hepatoprotective [9,10,11], neuroprotective [12], cardioprotective [13,14] and anticancer properties [15,16]. Andrographolide 1 (Figure 1) has been reported to be the main active compound in the plant [17,18]. Other minor diterpenoid lactones have been isolated from the plant, and their structures have been investigated [21] but their activities are not well studied. Studies about the pharmacological mechanism of its known anti-inflammatory actions have shown in 2004
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
