Quantitative determination of ambroxol in tablets by derivative UV spectrophotometric method and HPLC

Abstract

A derivative UV spectrophotometric method for the determination of ambroxol in tablets was developed. Determination of ambroxol in tablets was conducted by using first-order derivative UV spectrophotometric method at 255 nm ( n=5). Standards for the calibration graph ranging from 5.0 to 35.0 μg/ml were prepared from stock solution. The proposed method was accurate with 98.6±0.4% recovery value and precise with coefficient of variation (CV) of 1.22. These results were compared with those obtained by reference methods, zero-order UV spectrophotometric method and reversed-phase high-performance liquid chromatography (HPLC) method. A reversed-phase C 18 column with aqueous phosphate (0.01 M)–acetonitrile–glacial acetic acid (59:40:1, v/v/v) (pH 3.12) mobile phase was used and UV detector was set to 252 nm. Calibration solutions used in HPLC were ranging from 5.0 to 20.0 μg/ml. Results obtained by derivative UV spectrophotometric method was comparable to those obtained by reference methods, zero-order UV spectrophotometric method and HPLC, as far as ANOVA test, F calculated=0.762 and F theoretical=3.89, was concerned.