Abstract
The separation and determination of 3-nitropropanol (NPOH) and 3-nitropropionic acid (NPA) by reverse-phase high-pressure liquid chromatography (HPLC) is described. The system consists of a Micropak MCH-5 column eluted isocratically with 0.15% orthophosphoric acid (pH 2.0). This technique permits the simultaneous determination of NPA and NPOH in bovine and ovine plasma.
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