Abstract
Quick and accurate quantification of multiple biomarkers is quite a challenge in high throughput detection. Herein, we developed a novel lateral flow assay (LFA) based on Raman dyes (RDs) encoded core-shell surface enhanced Raman scattering (SERS) nanotags for rapid quantification of three cardiac biomarkers on a single test (T) line for early diagnosis of acute myocardial infarction (AMI). Owing to the signal amplification of encoded SERS nanotags and high surface area to volume ratio (SVR) of nitrocellulose (NC) membrane, rapid quantification of CK-MB, cTnI, and Myo with ultra-high sensitivity and wide linear dynamic range (LDR) was realized. We envision this method to find wide applications in in vitro diagnostics (IVD).
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