Abstract
Residual pesticides in agricultural production are one of the major food safety concerns around the world. In this study, we investigated the amount of isofenphos-methyl in corns by using surface-enhanced Raman spectroscopy (SERS) coupled with chemometric methods. The highest isofenphos-methyl peak at 1043 cm−1 was used to detect and quantify the amount of isofenphos-methyl spiked in methanol-water (1:1) solvent and corns. The limits of detection (LODs) of isofenphos-methyl in methanol-water solvent and corns were 0.01 μg/mL and 0.01 μg/g, respectively. Quantitative detection of isofenphos-methyl residues (0–5 μg/mL) was conducted using partial least squares (PLS) model. Based upon the results of leave-one-out cross-validation, isofenphos-methyl could be predicted by PLS (the correlation coefficient was 0.9964) with a low root mean square error of calibration (RMSEC=0.146). The developed SERS method provides a much simpler and more sensitive way to monitor isofenphos-methyl for safe production of corns, which can be extended to the other agricultural products.
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