Abstract
Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus mutans and dental caries. As S. mutans, is considered to be the principle etiological agent of dental caries, the development of a quick and convenient method for detection and quantification of these bacteria from patient saliva samples would simplify diagnosis and treatment. The purpose of this study was to compare a new means of quantifying bacteria using FTATM Elute cards and Real-Time Polymerase Chain Reaction to a conventional culture-based assay using oral S. mutans as a model sample. A total of 60 different saliva samples were investigated. The results show a significant negative correlation between the two methods, with a correlation coefficient of -0.577 (Spearman’s Correlation) and p
Highlights
Dental caries is one of the most common and costly diseases in the world, and rarely life threatening it is a major problem for health service providers [1]
The purpose of this study was to compare a new means of quantifying bacteria using FTATM Elute cards and Real-Time Polymerase Chain Reaction to a conventional culture-based assay using oral S. mutans as a model sample
The aim of this study was to compare a new means of quantifying bacteria using FTATM Elute cards and qPCR
Summary
Dental caries is one of the most common and costly diseases in the world, and rarely life threatening it is a major problem for health service providers [1]. Streptococcus mutans is considered a major etiologic agent of dental caries. Ous strains of oral streptococci using selective culturing media, such as Mitis-salivarius or Mitissalivarius-bacitracin agar, is complicated and time-consuming. A polymerase chain reaction (PCR) method for simple, rapid and reliable identification of S. mutans was developed [2,3]. Igarash et al utilized primers formulated from the dextranase (dexA) gene for the detection of S. mutans by PCR. Dextranase is an enzyme that hydrolyses glucans in plaque and is involved in the virulence of S. mutans [3]
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