Quantitative demonstration of the existence of two isoforms of canine hepcidin in the serum & urine of dogs

Abstract

Hepcidin-25 is the key peptide hormone controlling vertebrate iron metabolism. However, in the last twenty years there was some disagreement in the literature over the structure of this compound. The aim of this research was to study whether more than one isoform of canine hepcidin-25 exists. For the purpose of comparison serum concentrations of hepcidin-25 were determined in the samples of 47 dogs sick with acute/chronic inflammation too. The study demonstrated that two isoforms of canine hepcidin-25 exist. A statistical correlation may indicate that both molecules are synthesised by dogs together. No statistically significant correlations were found between the measured concentrations of the two canine hepcidin-25 isoforms and the measured serum iron parameters in the sampled dogs, irrespective of the measurements were made in serum or urine. The mean urinary total hepcidin-25/creatinine ratio in healthy dogs was 1.08 ± 0.10. The mean serum total hepcidin-25 concentration was 79.8 ± 4.9 ng/ml, about 65% of which was the 25β version. The presence of inflammation results in a statistically significant increase in the serum concentration of both hepcidin varieties. The role and fate of the two molecules may need to be researched further to provide better understanding of their relation.